PLXNB2 Monoclonal antibody, PBS Only

PLXNB2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G9B2

Cat no : 67265-1-PBS

Synonyms

dJ402G11.3, KIAA0315, MM1, Nbla00445, PLEXB2, plexin B2, PLXNB2

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Validation Data Gallery

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  WB analysis using 67265-1-Ig (same clone as 67265-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

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  WB analysis of ATDC-5 using 67265-1-Ig (same clone as 67265-1-PBS)

  ATDC-5 cells were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67265-1-Ig (same clone as 67265-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67265-1-Ig (same clone as 67265-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67265-1-Ig (same clone as 67265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67265-1-Ig (PLXNB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67265-1-Ig (same clone as 67265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67265-1-Ig (PLXNB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

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Product Information

67265-1-PBS targets PLXNB2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLXNB2 fusion protein Ag28706
• Full Name: plexin B2
• Calculated Molecular Weight: 205 kDa
• Observed Molecular Weight: 240 kDa
• GenBank Accession Number: NM_012401
• Gene Symbol: PLXNB2
• Gene ID (NCBI): 23654
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 20-466aa of human plexin B2 can recognize precursor and alpha subunit of plexin B2.