PLXDC2 Polyclonal antibody

PLXDC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 12285-1-AP

Synonyms

UNQ2514/PRO6003, Tumor endothelial marker 7-related protein, TEM7R, Plexin domain-containing protein 2, plexin domain containing 2

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Validation Data Gallery

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  WB analysis of A431 using 12285-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12285-1-AP (PLXDC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12285-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12285-1-AP (PLXDC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12285-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12285-1-AP (PLXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 12285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12285-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12285-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, MKN-45 cells
• Positive IHC detected in: human lung cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

12285-1-AP targets PLXDC2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLXDC2 fusion protein Ag2940
• Full Name: plexin domain containing 2
• Calculated Molecular Weight: 480 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC012885
• Gene Symbol: PLXDC2
• Gene ID (NCBI): 84898
• RRID: AB_2166239
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLXDC2 (Plexin-domain containing 2), also named tumor endothelial marker 7-related protein (TEM7R), is a single-pass type I membrane protein with some homology to nidogen and to plexins. PLXDC2 is located in tumor endothelium and plays a role in tumor angiogenesis. Three isoforms of PLXDC2 produced by alternative splicing have been described. Recent finding revealed that PLXDC2 is a mitogen for neural progenitors and functions as a novel regulator of the development of different brain regions, suggesting its role in coordinating proliferation and differentiation in the developing nervous system.

Publications

Species	Application	Title
human	IHC,IF	Front Cell Dev Biol; Overexpression of PLXDC2 in Stromal Cell-Associated M2 Macrophages Is Related to EMT and the Progression of Gastric Cancer.; Authors - Yiming Guan
human	WB,IHC	Cancer Res Commun; Adaptive c-Met-PLXDC2 Signaling Axis Mediates Cancer Stem Cell Plasticity to Confer Radioresistance-associated Aggressiveness in Head and Neck Cancer; Authors - Liwei Lang
human,mouse	WB	Dev Cell; Secreted PTEN binds PLXDC2 on macrophages to drive antitumor immunity and tumor suppression; Authors - Cheng Zhang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (09-05-2021)

This antibody detects two bands around 55 kd, as expected molecular weight. However, neither of the bands are sensitive to deglycosylation treatment, suggesting these bands are from some other proteins other than Plxdc2. According to literature, Plxdc2 is glycosylated.

• Applications: Western Blot