Anti-Mouse PLVAP (MECA-32)

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  IF Staining of mouse small intestine using 65214-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using PLVAP antibody (65214-1-Ig, Clone: MECA-32 ) at dilution of 1:250 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of bEnd.3 using 65214-1-Ig

  1X10^6 bEnd.3 cells were surface stained with 0.5 ug Anti-Mouse Plvap (65214-1-Ig, Clone:MECA-32) (red), or 0.5 ug Isotype Control. Cells were not fixed.

PLVAP Monoclonal Antibody for IF-P, FC

Cat No. 65214-1-Ig

Clone No.MECA-32

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

IF-P, FC

Synonyms

MECA-32, Panendothelial cell antigen, Plasmalemma vesicle protein 1, Plvap, PV 1

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Tested Applications

• Positive IF-P detected in: mouse small intestine tissue
• Positive FC detected in: bEnd.3 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:125-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65214-1-Ig targets PLVAP in IF-P, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse lymph node stromal cells
• Full Name: plasmalemma vesicle associated protein
• GenBank Accession Number: BC013517
• Gene Symbol: Plvap
• Gene ID (NCBI): 84094
• RRID: AB_3084905
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q91VC4
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Plasmalemma vesicle-associated protein (PLVAP), also known as PV-1, PAL-E and MECA-32 antigen, is an endothelial cell-specific type II membrane N-glycosylated glycoprotein with a molecular weight of approximately 50-65 kDa, that forms homodimers in situ (PMID: 7626790; 30231925). The MECA-32 antibody, derived from rats immunized with murine lymph node stroma, shows high specificity for mouse endothelium in both embryonic and mature tissues (PMID: 2683264; 7626790). It recognizes most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where the MECA-32 antigen has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier (PMID: 7626790).