PLTP Recombinant antibody

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  WB analysis using 84592-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84592-1-RR (PLTP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 84592-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84592-1-RR (PLTP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84592-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84592-1-RR (PLTP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84592-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84592-1-RR against Human PLTP were performed. The affinity constant is below 1 pM.

PLTP Recombinant Antibody for WB, IHC, ELISA

Cat No. 84592-1-RR

Clone No.241583F3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Phospholipid transfer protein, Lipid transfer protein II, 241583F3

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84592-1-RR targets PLTP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PLTP fusion protein Ag8171
• Full Name: phospholipid transfer protein
• Calculated Molecular Weight: 493 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC005045
• Gene Symbol: PLTP
• Gene ID (NCBI): 5360
• RRID: AB_3672088
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P55058
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLTP (phospholipid transfer protein) belongs to the family of lipid-binding and lipid-transfer proteins. PLTP is involved in the distribution and metabolism of high-density lipoprotein ( HDL ) and reverse cholesterol transfer ( RCT ). In plasma, PLTP catalyzes the transfer of phospholipids ( mainly phosphatidylcholine ) between lipoproteins (PMID: 29558025). PLTP has a predicted protein molecular mass of approximately 55 kDa, and molecular weights of 70-80 kDa can also be detected in some literature (PMID: 26160914; 36312255; 29680823; 15342679; 7654777).