PLSCR1 Recombinant antibody

• 

  WB analysis using 84888-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84888-4-RR (PLSCR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84888-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84888-4-RR against Human PLSCR1 were performed. The affinity constant is 0.377 nM.

PLSCR1 Recombinant Antibody for WB, ELISA

Cat No. 84888-4-RR

Clone No.242372B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

phospholipid scramblase 1, MMTRA1B, Mg(2+)-dependent nuclease, EC:3.1.-.-, Ca(2+)-dependent phospholipid scramblase 1

---

Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, A549 cells, MCF-7 cells, Jurkat cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84888-4-RR targets PLSCR1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PLSCR1 fusion protein Ag2172
• Full Name: phospholipid scramblase 1
• Calculated Molecular Weight: 318 aa, 35 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC021100
• Gene Symbol: PLSCR1
• Gene ID (NCBI): 5359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O15162
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLSCR1 (Phospholipid scramblase 1) is a member of the family of membrane proteins that mediate the transbilayer movement of phospholipids ("scrambling") in a Ca2+ dependent manner, which was identified as an a type II transmembrane protein involved in the calcium-dependent, nonspecific, rapid redistribution of phospholipids. A cysteine-rich palmitoylation motif [184CCCPCC189] of PLSCR1, a thioester linkage to the sulfhydryl groups of cysteines, controls the transportation of PLSCR1 to the cell membrane or nucleus (PMID: 32292520). PLSCR1 is strongly expressed in response to interferon (IFN) treatment and viral infection (PMID: 35138119, 23487022).