PLOD3 Polyclonal antibody

• Featured Product
• KD/KO Validated

PLOD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

34

Cat no : 11027-1-AP

Synonyms

Procollagen glucosyltransferase, Procollagen galactosyltransferase, Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3, Lysyl hydroxylase 3, LH3

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Validation Data Gallery

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  WB analysis using 11027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11027-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11027-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11027-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 11027-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11027-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11027-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11027-1-AP

  IP result of anti-PLOD3 (IP:11027-1-AP, 3ug; Detection:11027-1-AP 1:500) with HepG2 cells lysate 6000ug.

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  IHC staining of human pancreas cancer using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11027-1-AP (PLOD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11027-1-AP (PLOD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11027-1-AP (PLOD3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11027-1-AP (PLOD3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11027-1-AP

  Immunofluorescent analysis of HepG2 cells using 11027-1-AP (PLOD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HepG2 using 11027-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PLOD3 antibody (11027-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, PC-3 cells, HepG2 cells, HeLa cells, human placenta tissue, mouse placenta tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human pancreas cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 25 publications below
• IHC: See 6 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Product Information

11027-1-AP targets PLOD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLOD3 fusion protein Ag1480
• Full Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
• Calculated Molecular Weight: 738 aa, 85 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC011674
• Gene Symbol: PLOD3
• Gene ID (NCBI): 8985
• RRID: AB_2165781
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.

Publications

Species	Application	Title
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
		Nat Commun; Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3.; Authors - Luigi Scietti
human	WB,IF	Nat Commun; Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis.; Authors - Blerida Banushi
human	WB	Am J Hum Genet; A connective tissue disorder caused by mutations of the lysyl hydroxylase 3 gene.; Authors - Salo Antti M AM
mouse	WB	PLoS Genet; Abnormal type I collagen post-translational modification and crosslinking in a cyclophilin B KO mouse model of recessive osteogenesis imperfecta.; Authors - Wayne A Cabral
human	WB	Matrix Biol; Mutations in PLOD3, encoding lysyl hydroxylase 3, cause a complex connective tissue disorder including recessive dystrophic epidermolysis bullosa-like blistering phenotype with abnormal anchoring fibrils and type VII collagen deficiency.; Authors - Hassan Vahidnezhad

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