PLOD2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PLOD2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgA

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H9E1

Cat no : 66342-1-PBS

Synonyms

LH2, Lysyl hydroxylase 2, PLOD2, TLH

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Validation Data Gallery

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  WB analysis using 66342-1-Ig (same clone as 66342-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66342-1-Ig (PLOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66342-1-Ig (same clone as 66342-1-PBS)

  WB result of PLOD2 antibody (66342-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLOD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

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  WB analysis of DU 145 using 66342-1-Ig (same clone as 66342-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 66342-1-Ig (PLOD2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66342-1-Ig (same clone as 66342-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66342-1-Ig (PLOD2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66342-1-Ig (same clone as 66342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66342-1-Ig (same clone as 66342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66342-1-Ig (same clone as 66342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66342-1-Ig (same clone as 66342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

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Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Product Information

66342-1-PBS targets PLOD2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgA
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLOD2 fusion protein Ag5779
• Full Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
• Calculated Molecular Weight: 758 aa, 85 kDa
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC037169
• Gene Symbol: PLOD2
• Gene ID (NCBI): 5352
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy.(PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.