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PLOD2-Specific Polyclonal antibody

PLOD2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 21214-1-AP

Synonyms

PLOD2, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2, PLOD2/LH2, Lysyl hydroxylase 2, EC:1.14.11.4



Tested Applications

Positive WB detected inCaco-2 cells, A431 cells, DU 145 cells, HT-1080 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman liver cancer tissue, human pancreas tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21214-1-AP targets PLOD2-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rabbit, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLOD2-Specific fusion protein Ag15614
Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
Calculated Molecular Weight 758 aa, 85 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC037169
Gene Symbol PLOD2
Gene ID (NCBI) 5352
RRIDAB_10733347
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.

Protocols

Product Specific Protocols
WB protocol for PLOD2-Specific antibody 21214-1-APDownload protocol
IHC protocol for PLOD2-Specific antibody 21214-1-APDownload protocol
IF protocol for PLOD2-Specific antibody 21214-1-APDownload protocol
IP protocol for PLOD2-Specific antibody 21214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

J Clin Invest

Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma.

Authors - Yulong Chen
zebrafishWB

Cancer Res

Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis

Authors - Ying Liu
human, mouseWB

Cancer Discov

Hypoxia-Dependent Modification of Collagen Networks Promotes Sarcoma Metastasis.

Authors - Eisinger-Mathason T S Karin TS
mouseWB

Arthritis Rheumatol

Therapeutic effect of cyclin-dependent kinase 4/6 inhibitor on dermal fibrosis in murine models of systemic sclerosis.

Authors - Akio Yamamoto
humanWB

Proc Natl Acad Sci U S A

Intratumoral oxygen gradients mediate sarcoma cell invasion.

Authors - Daniel M Lewis
mouseIHC

Arthritis Rheum

Gene expression analysis of osteoarthritis synovium reveals elevation of transforming growth factor-beta responsive genes in osteoarthritis-related fibrosis.

Authors - Remst D F G DF

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

RICHA (Verified Customer) (12-02-2019)

Excellent

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse fibroblast