PLOD2-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

PLOD2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

50

Reviews (1)

Cat no : 21214-1-AP

Synonyms

PLOD2, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2, PLOD2/LH2, Lysyl hydroxylase 2, EC:1.14.11.4

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Validation Data Gallery

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  WB analysis of A431 using 21214-1-AP

  WB result of PLOD2-Specific antibody (21214-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLOD2-Specific transfected A431 cells.

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  WB analysis using 21214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21214-1-AP

  DU 145 cells lysates were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 21214-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 21214-1-AP

  IP result of anti-PLOD2-Specific (IP:21214-1-AP, 4ug; Detection:21214-1-AP 1:500) with mouse testis tissue lysate 5000ug.

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  IHC staining of human liver cancer using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 21214-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLOD2-Specific antibody (21214-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 21214-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PLOD2-Specific (21214-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Caco-2 cells, A431 cells, DU 145 cells, HT-1080 cells, HEK-293 cells, HeLa cells, HepG2 cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human liver cancer tissue, human pancreas tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 36 publications below
• IHC: See 21 publications below
• IF: See 7 publications below

Product Information

21214-1-AP targets PLOD2-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rabbit, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLOD2-Specific fusion protein Ag15614
• Full Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
• Calculated Molecular Weight: 758 aa, 85 kDa
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC037169
• Gene Symbol: PLOD2
• Gene ID (NCBI): 5352
• RRID: AB_10733347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.

Publications

Species	Application	Title
human	WB, IHC	J Clin Invest; Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma.; Authors - Yulong Chen
zebrafish	WB	Cancer Res; Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis; Authors - Ying Liu
human, mouse	WB	Cancer Discov; Hypoxia-Dependent Modification of Collagen Networks Promotes Sarcoma Metastasis.; Authors - Eisinger-Mathason T S Karin TS
mouse	WB	Arthritis Rheumatol; Therapeutic effect of cyclin-dependent kinase 4/6 inhibitor on dermal fibrosis in murine models of systemic sclerosis.; Authors - Akio Yamamoto
human	WB	Proc Natl Acad Sci U S A; Intratumoral oxygen gradients mediate sarcoma cell invasion.; Authors - Daniel M Lewis
mouse	IHC	Arthritis Rheum; Gene expression analysis of osteoarthritis synovium reveals elevation of transforming growth factor-beta responsive genes in osteoarthritis-related fibrosis.; Authors - Remst D F G DF

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

RICHA (Verified Customer) (12-02-2019)

Excellent

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse fibroblast