PLK3 Polyclonal antibody

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  WB analysis using 30486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30486-1-AP (PLK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 30486-1-AP

  IP result of anti-PLK3 (IP:30486-1-AP, 4ug; Detection:30486-1-AP 1:500) with HeLa cells lysate 1750 ug.

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  IHC staining of human colon using 30486-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30486-1-AP (PLK3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PLK3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 30486-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

PLK 3, FNK, FGF-inducible kinase, EC:2.7.11.21, Cytokine-inducible serine/threonine-protein kinase

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Tested Applications

• Positive WB detected in: HEK-293T cells, K-562 cells, NCI-H1299 cells, U2OS cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30486-1-AP targets PLK3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLK3 fusion protein Ag33186
• Full Name: polo-like kinase 3 (Drosophila)
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC013899
• Gene Symbol: PLK3
• Gene ID (NCBI): 1263
• RRID: AB_3669726
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H4B4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLK3(Polo-like kinase 3) is also named as CNK, FNK, PRK and belongs to the protein kinase superfamily. It is involved in the regulation of DNA damage checkpoint as well as in M-phase function. Plk3 physically interacts with p53 and phosphorylates this tumor suppressor protein on serine-20, suggesting that the role of Plk3 in cell cycle progression is mediated, at least in part, through direct regulation of p53(PMID:12548019).