PLK3 Polyclonal antibody
PLK3 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 30486-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293T cells, K-562 cells, NCI-H1299 cells, U2OS cells |
Positive IP detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
30486-1-AP targets PLK3 in WB, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PLK3 fusion protein Ag33186 |
Full Name | polo-like kinase 3 (Drosophila) |
Calculated Molecular Weight | 72 kDa |
Observed Molecular Weight | 72 kDa |
GenBank Accession Number | BC013899 |
Gene Symbol | PLK3 |
Gene ID (NCBI) | 1263 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PLK3(Polo-like kinase 3) is also named as CNK, FNK, PRK and belongs to the protein kinase superfamily. It is involved in the regulation of DNA damage checkpoint as well as in M-phase function. Plk3 physically interacts with p53 and phosphorylates this tumor suppressor protein on serine-20, suggesting that the role of Plk3 in cell cycle progression is mediated, at least in part, through direct regulation of p53(PMID:12548019).
Protocols
Product Specific Protocols | |
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WB protocol for PLK3 antibody 30486-1-AP | Download protocol |
IP protocol for PLK3 antibody 30486-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |