Phospho-PLK1 (Ser326) Polyclonal antibody

Phospho-PLK1 (Ser326) Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 19553-1-AP

Synonyms

Phospho-PLK1 (Ser326), phosphorylation PLK1 SER326, PLK, PLK 1, PLK1, Polo like kinase 1, STPK13

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Validation Data Gallery

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  WB analysis of MCF-7 using 19553-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19553-1-AP (PLK1-phospho(S326) antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 19553-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19553-1-AP (PLK1-phospho(S326) antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 19553-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19553-1-AP (PLK1-phospho(S326) antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

19553-1-AP targets Phospho-PLK1 (Ser326) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: polo-like kinase 1 (Drosophila)
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: NM_005030
• Gene Symbol: PLK1
• Gene ID (NCBI): 5347
• RRID: AB_2878589
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLK1, also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. The antibody is specific to Ser(326)-phospho.

Publications

Species	Application	Title
rat	WB	Toxicol Lett; Oxcarbazepine induces mitotic catastrophe and apoptosis in NRK-52E proximal tubular cells.; Authors - Momoka Ota
human,mouse	WB	Arch Biochem Biophys; Homoharringtonine inhibits melanoma cells proliferation in vitro and vivo by inducing DNA damage, apoptosis, and G2/M cell cycle arrest.; Authors - Jia-Feng Tang