Perilipin 1 Recombinant antibody

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  WB analysis using 83905-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83905-4-RR (PLIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83905-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83905-4-RR against Human Perilipin 1 were performed. The affinity constant is 0.617 nM.

Perilipin 1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83905-4-RR

Clone No.240994H1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PLIN, PLIN1, PERI, 240994H1

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Tested Applications

• Positive WB detected in: MCF-7 cells, MDA-MB-231 cells, 3T3-L1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83905-4-RR targets Perilipin 1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Perilipin 1 fusion protein Ag26426
• Full Name: perilipin
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: NM_001145311
• Gene Symbol: Perilipin 1
• Gene ID (NCBI): 5346
• RRID: AB_3671486
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O60240
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLIN1, also known as Perilipin 1, PLIN, PERI, belongs to the perilipin family. PLIN1 is a hormonally-regulated phosphoprotein that encircles the lipid storage droplet in adipocytes and modulates adipocyte lipid metabolism (PMID: 2040638). PLIN1 is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in inhibiting lipolysis. PLIN1 is detected in adipocytes of white adipose tissue, visceral adipose tissue, and mammary gland (PMID: 27832861, 9521880). The deficiency of PLIN1 causes lipodystrophic syndromes, which disables the optimal accumulation of triglycerides in adipocytes that results in abnormal deposition of lipids in tissues such as skeletal muscle and liver. The storage of lipids in the liver leads to insulin resistance and hypertriglyceridemia (PMID: 21345103).