PLEKHB2 Monoclonal antibody

PLEKHB2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H9D7

Cat no : 67877-1-Ig

Synonyms

Evectin 2, EVT2, FLJ20783, FLJ23679, FLJ30436, PLEKHB2

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Validation Data Gallery

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  WB analysis using 67877-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67877-1-Ig (PLEKHB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Tested Applications

• Positive WB detected in: Caco-2 cells, HT-29 cells, COLO320 cells, SW480 cells, NCCIT cells, BxPC-3 cells, U-87 MG cells, HL-60 cells, Pig colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67877-1-Ig targets PLEKHB2 in WB, ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLEKHB2 fusion protein Ag9231
• Full Name: pleckstrin homology domain containing, family B (evectins) member 2
• Calculated Molecular Weight: 222 aa, 25 kDa
• Observed Molecular Weight: 19-25;30-34 kDa
• GenBank Accession Number: BC013991
• Gene Symbol: PLEKHB2
• Gene ID (NCBI): 55041
• RRID: AB_2918634
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLEKHB2, also named as EVT2 (Evectin-2), is involved in retrograde transport of recycling endosomes. PLEKHB2 has been identified as post-Golgi proteins of unknown function that have a PH domain that typically binds polyphosphoinositides. PLEKHB2 is expressed in a broad range of tissues. PLEKHB2 has some isoforms with MW 19-26 kDa and 34 kDa. This antibody recognizes all the isoforms of PLEKHB2.