PLEKHA2 Polyclonal antibody

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  WB analysis using 32169-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32169-1-AP (PLEKHA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 32169-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PLEKHA2 antibody (32169-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HeLa using 32169-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PLEKHA2 antibody (32169-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

PLEKHA2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 32169-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

TAPP-2, TAPP2, Tandem PH domain-containing protein 2, Pleckstrin homology domain-containing family A member 2, Pleckstrin Homology Domain Containing A2

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Tested Applications

• Positive WB detected in: HeLa cells, JurKat cells, RT-4 cells
• Positive IF/ICC detected in: HeLa cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32169-1-AP targets PLEKHA2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLEKHA2 fusion protein Ag36596
• Full Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 2
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC166639
• Gene Symbol: PLEKHA2
• Gene ID (NCBI): 59339
• RRID: AB_3670213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9HB19
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLEKHA2 (Pleckstrin homology domain containing A2), also known as TAPP2, is a member of the PLEKHA family of proteins.PLEKHA2 is a junctional protein with a Pleckstrin homology domain that is involved in signaling after lymphocyte activation and plays an important role in cytoskeletal reorganization driven by phosphatidylinositol 3-kinase.