PLEKHA1 Polyclonal antibody

PLEKHA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10238-1-AP

Synonyms

TAPP-1, TAPP1, TAPP 1, Tandem PH domain-containing protein 1, Pleckstrin homology domain-containing family A member 1

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Validation Data Gallery

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  WB analysis of human placenta using 10238-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10238-1-AP (PLEKHA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 10238-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10238-1-AP (PLEKHA1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 10238-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10238-1-AP (PLEKHA1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 10238-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10238-1-AP (PLEKHA1 antibody) at dilution of 1:100 (under 25x lens).

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  IF Staining of HeLa using 10238-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 10238-1-AP (PLEKHA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human placenta tissue, Jurkat cells
• Positive IHC detected in: human colon tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

10238-1-AP targets PLEKHA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLEKHA1 fusion protein Ag0318
• Full Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC001136
• Gene Symbol: PLEKHA1
• Gene ID (NCBI): 59338
• RRID: AB_2163993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pleckstrin homology (PH) domain is commonly found in eukaryotic signaling proteins and possesses multiple functions including the abilities to bind inositol phosphates and various proteins. The tandem PH domain containing protein-1 (TAPP1) or PH domain containing-family A (phosphoinositide binding specific) member 1 (PLEKHA1), interacts strongly and specifically with phosphatidylinositol 3,4-trisphosphate [PtdIns(3,4)P(2)], which is one of the immediate breakdown products of PtdIns(3,4,5) P (3) and functions as a signalling molecule in insulin- and growth-factor-stimulated pathways. TAPP1 is also associated with the protein- tyrosine-phosphatase-like protein-1 (PTPL1 also known as FAP-1) and maintains PTPL1 in cytoplasm. By binding to PtdIns(3,4) P (2) and PTPL1, TAPP1 may regulate the membrane localization of PTPL1."

Publications

Species	Application	Title
mouse	WB	J Exp Med; Secretogranin III as a disease-associated ligand for antiangiogenic therapy of diabetic retinopathy.; Authors - Michelle E LeBlanc
mouse		Exp Eye Res; Function-first ligandomics for ocular vascular research and drug target discovery.; Authors - Xin Rong
human	WB	J Proteome Res; EIF4G2 Promotes Hepatocellular Carcinoma Progression via IRES-dependent PLEKHA1 Translation Regulation; Authors - Manman Li