PLEK Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PLEK Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D5C2

Cat no : 66431-1-PBS

Synonyms

P47, Platelet 47 kDa protein, pleckstrin, PLEK

---

Validation Data Gallery

• 

  WB analysis of U-937 using 66431-1-Ig (same clone as 66431-1-PBS)

  WB result of PLEK antibody (66431-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLEK transfected U-937 cells. This data was developed using the same antibody clone with 66431-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 66431-1-Ig (same clone as 66431-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66431-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 66431-1-Ig (same clone as 66431-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66431-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 66431-1-Ig (same clone as 66431-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66431-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 66431-1-Ig (same clone as 66431-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66431-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66431-1-Ig (same clone as 66431-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66431-1-Ig (PLEK Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66431-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66431-1-Ig (same clone as 66431-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66431-1-Ig (PLEK Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66431-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 66431-1-Ig (same clone as 66431-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using PLEK antibody (66431-1-Ig, Clone: 1D5C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66431-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 66431-1-Ig (same clone as 66431-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using PLEK antibody (66431-1-Ig, Clone: 1D5C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66431-1-PBS in a different storage buffer formulation.

---

Product Information

66431-1-PBS targets PLEK in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig, mouse samples.

• Tested Reactivity: human, pig, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLEK fusion protein Ag3187
• Full Name: pleckstrin
• Calculated Molecular Weight: 350 aa, 40 kDa
• Observed Molecular Weight: 40-47 kDa
• GenBank Accession Number: BC018549
• Gene Symbol: PLEK
• Gene ID (NCBI): 5341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)