PLEC Polyclonal antibody

PLEC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 29170-1-AP

Synonyms

PLEC1, Plectin, PLTN, Plectin 1, PLEC1b

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Validation Data Gallery

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  WB analysis using 29170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29170-1-AP (PLEC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 29170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29170-1-AP (PLEC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 29170-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PLEC antibody (29170-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, mouse kidney tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29170-1-AP targets PLEC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLEC fusion protein Ag29418
• Full Name: plectin 1, intermediate filament binding protein 500kDa
• Calculated Molecular Weight: 532 kDa
• Observed Molecular Weight: 510 kDa
• GenBank Accession Number: NM_201380
• Gene Symbol: Plectin
• Gene ID (NCBI): 5339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011)

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marina (Verified Customer) (03-22-2024)

Samples are two tumour (glioblastoma) cell lines (TC1, TC2). Gradient gel 4-15 %. Dilution 1:2000, primary antibody incubation 4ºC overnight, secondary antibody incubation 1 h room temperature. The blot was incubated with anti-plectin antibody after incubation with anti-spectrin beta chain (ref. 67978-1), stripping and re-blocking. The bands appear at the expected size of the target protein plectin (exposure 2 minutes).

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human tumour cell lines

PLEC Antibody Western Blot validation (1:2000 dilution) in Human tumour cell lines (Cat no:29170-1-AP)