Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 17327-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 17327-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 17327-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human stomach using 17327-1-AP
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17327-1-AP (PLD3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17327-1-AP (PLD3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 17327-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 17327-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of A431 using 17327-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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HEK-293 cells, HeLa cells, HepG2 cells, SH-SY5Y cells
Positive IHC detected in
human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse brain tissue
Positive IF/ICC detected in
A431 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:3000
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 17327-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 17327-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 17327-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human stomach using 17327-1-AP
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17327-1-AP (PLD3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of mouse brain using 17327-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 17327-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF/ICC Figures
IF Staining of A431 using 17327-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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