PLD1 Polyclonal antibody, PBS Only

PLD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18355-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 18355-1-AP (same clone as 18355-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18355-1-AP (PLD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18355-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 18355-1-AP (same clone as 18355-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18355-1-AP (PLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18355-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18355-1-AP (same clone as 18355-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PLD1 antibody (18355-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18355-1-PBS in a different storage buffer formulation.

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Product Information

18355-1-PBS targets PLD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLD1 fusion protein Ag13227
• Full Name: phospholipase D1, phosphatidylcholine-specific
• Calculated Molecular Weight: 1074 aa, 124 kDa
• Observed Molecular Weight: 112-124 kDa
• GenBank Accession Number: BC068976
• Gene Symbol: PLD1
• Gene ID (NCBI): 5337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PLD1(Phospholipase D1) belongs to the phospholipase D family. It is implicated in the release process of a variety of hormones, most likely at a late stage of exocytosis through the production of lipids that are essential for the fusion process and seem to be also essential for fast neurotransmitter release. PLD1 is primarily localized in vesicular structures such as endosomes, lysosomes, and autophagosomes.