PLCL2 Monoclonal antibody, PBS Only

PLCL2 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse, Pig

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C8A4

Cat no : 67471-1-PBS

Synonyms

FLJ13484, KIAA1092, Phospholipase C epsilon 2, Phospholipase C L2, phospholipase C like 2, PLC epsilon 2, PLC L(2), PLC L2, PLCE2, PLCL2

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Validation Data Gallery

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  WB analysis using 67471-1-Ig (same clone as 67471-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67471-1-Ig (PLCL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67471-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67471-1-Ig (same clone as 67471-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67471-1-Ig (PLCL2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67471-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67471-1-PBS targets PLCL2 in WB, IF, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse, Pig samples.

• Tested Reactivity: Human, Rat, Mouse, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLCL2 fusion protein Ag11461
• Full Name: phospholipase C-like 2
• Calculated Molecular Weight: 126 kDa
• Observed Molecular Weight: 126 kDa
• GenBank Accession Number: BC036392
• Gene Symbol: PLCL2
• Gene ID (NCBI): 23228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PLCL2, a novel phospholipase C-like protein, is expressed in lymphocytes and platelets. The expression of PLCL2 is associated with the proliferation of mature B cells in the immune system. PLCL2 is a new susceptibility loci for myocardial infarction. It has been shown that PLCL2 plays a key role in the pathogenesis of atherosclerosis and systemic sclerosisit. There are three isoforms of PLCL2 protein and 67471-1-Ig antibody detects the 126 kDa band in SDS-PAGE. (PMID: 24916648, 25880423)