PLCG2 Polyclonal antibody

PLCG2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29567-1-AP

Synonyms

Phospholipase C-gamma-2, Phospholipase C IV, Phospholipase C gamma 2, Phosphoinositide phospholipase C-gamma-2, EC:3.1.4.11

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Validation Data Gallery

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  WB analysis using 29567-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29567-1-AP (PLCG2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of U-937 using 29567-1-AP

  Immunofluorescent analysis of un-fixed Live U-937 cells using PLCG2 antibody (29567-1-AP) at dilution of 1:100 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, Ramos cells
• Positive IF/ICC detected in: Live U-937 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29567-1-AP targets PLCG2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phospholipase C, gamma 2 (phosphatidylinositol-specific)
• Calculated Molecular Weight: 148 kDa
• Observed Molecular Weight: 148 kDa
• GenBank Accession Number: BC007565
• Gene Symbol: PLCG2
• Gene ID (NCBI): 5336
• RRID: AB_2918326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospholipase C-gamma2 (PLCG2) is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor.IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.