PLCG2 Polyclonal antibody

PLCG2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27173-1-AP

Synonyms

Phospholipase C gamma 2, Phospholipase C IV, PLC gamma 2, PLC IV, PLC γ2, PLCG2, PLCγ2

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Validation Data Gallery

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  WB analysis using 27173-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27173-1-AP (PLCG2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 27173-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27173-1-AP (PLCG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27173-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27173-1-AP (PLCG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, Ramos cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27173-1-AP targets PLCG2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLCG2 fusion protein Ag25847
• Full Name: phospholipase C, gamma 2 (phosphatidylinositol-specific)
• Calculated Molecular Weight: 148 kDa
• Observed Molecular Weight: 148 kDa
• GenBank Accession Number: BC007565
• Gene Symbol: PLCG2
• Gene ID (NCBI): 5336
• RRID: AB_2880785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.