PLCD4 Polyclonal antibody

PLCD4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10589-2-AP

Synonyms

hPLCD4, Phospholipase C delta 4, phospholipase C, delta 4, PLC delta 4, PLCD4

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 10589-2-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10589-2-AP (PLCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10589-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10589-2-AP (PLCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10589-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10589-2-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10589-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10589-2-AP (PLCD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10589-2-AP targets PLCD4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLCD4 fusion protein Ag0937
• Full Name: phospholipase C, delta 4
• Calculated Molecular Weight: 85-90 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC006355
• Gene Symbol: PLCD4
• Gene ID (NCBI): 84812
• RRID: AB_2163398
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLCD4 is a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4,5-bisphosphate into two intracellular second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer.