PLCB3 Monoclonal antibody, PBS Only

PLCB3 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B8B3

Cat no : 66668-1-PBS

Synonyms

FLJ37084, Phospholipase C beta 3, PLC beta 3, PLCB3

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Validation Data Gallery

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  WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

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  WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

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  WB analysis of Sp2/0 using 66668-1-Ig (same clone as 66668-1-PBS)

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66668-1-Ig (same clone as 66668-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66668-1-Ig (same clone as 66668-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66668-1-Ig (same clone as 66668-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66668-1-Ig (same clone as 66668-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66668-1-Ig (same clone as 66668-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66668-1-Ig (same clone as 66668-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66668-1-Ig (same clone as 66668-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66668-1-PBS targets PLCB3 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLCB3 fusion protein Ag15845
• Full Name: phospholipase C, beta 3 (phosphatidylinositol-specific)
• Calculated Molecular Weight: 1234 aa, 139 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC142681
• Gene Symbol: PLCB3
• Gene ID (NCBI): 5331
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq/11 and/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.