PLCB3 Polyclonal antibody

PLCB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21370-1-AP

Synonyms

Phospholipase C beta 3, PLC beta 3, PLCB3

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Validation Data Gallery

• 

  WB analysis of MCF-7 using 21370-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 21370-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 21370-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 21370-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 21370-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 21370-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21370-1-AP (PLCB3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 21370-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21370-1-AP (PLCB3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: MCF-7 cells, A431 cells, COLO 320 cells, HEK-293T cells, NIH/3T3 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21370-1-AP is PLCB3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLCB3 fusion protein Ag15845
• Full Name: phospholipase C, beta 3 (phosphatidylinositol-specific)
• Calculated Molecular Weight: 1234 aa, 139 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC142681
• Gene Symbol: PLCB3
• Gene ID (NCBI): 5331
• RRID: AB_2878848
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.