PLAC8 Polyclonal antibody

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  WB analysis of human peripheral blood leukocyte using 12284-1-AP

  human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 12284-1-AP (PLAC8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 12284-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12284-1-AP (PLAC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12284-1-AP (PLAC8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12284-1-AP (PLAC8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12284-1-AP (PLAC8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12284-1-AP (PLAC8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12284-1-AP (PLAC8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12284-1-AP (PLAC8 antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

PLAC8 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12284-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

Protein C15, Placenta-specific gene 8 protein, placenta 8, onzin, C15

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Tested Applications

• Positive WB detected in: human peripheral blood leukocyte, PANC-1 cells
• Positive IHC detected in: human placenta tissue, human brain tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

12284-1-AP targets PLAC8 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLAC8 fusion protein Ag2939
• Full Name: placenta-specific 8
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC012205
• Gene Symbol: PLAC8
• Gene ID (NCBI): 51316
• RRID: AB_11182285
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NZF1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLAC8, also named as Protein C15, is a placenta-specific protein. The expression was downregulated upon activation, particularly in dendritic cells generated in vitro from CD34 (142230) progenitor cells. The MW of PLAC8 is about 12-20kd.

Publications

Species	Application	Title
human	IHC	FASEB J; PLAC8 promotes the autophagic activity and improves the growth priority of human trophoblast cells.; Authors - Xuan Feng
human	IHC	Int J Med Sci; Expression profiling of lncRNAs and mRNAs in placental site trophoblastic tumor (PSTT) by microarray.; Authors - Jianfeng Gan
human	WB,IHC	Histochem Cell Biol; PLAC8 contributes to the malignant behaviors of cervical cancer cells by activating the SOX4-mediated AKT pathway; Authors - Boya Deng ; KD Validated
human	IF	PLoS Genet; Single-cell RNA profiling reveals classification and characteristics of mononuclear phagocytes in colorectal cancer; Authors - Tiantian Ji