PAFAH Polyclonal antibody

• Featured Product
• KD/KO Validated

PAFAH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 15526-1-AP

Synonyms

PLA2G7, gVIIA-PLA2, gVIIA PLA2, Group-VIIA phospholipase A2, EC:3.1.1.47

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Validation Data Gallery

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  WB analysis of RAW264.7 using 15526-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 15526-1-AP (PAFAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 15526-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 15526-1-AP (PAFAH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 15526-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15526-1-AP (PAFAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15526-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15526-1-AP (PAFAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 15526-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15526-1-AP (PAFAH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15526-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15526-1-AP (PAFAH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15526-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15526-1-AP (PAFAH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15526-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15526-1-AP (PAFAH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of RAW 264.7 using 15526-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed RAW 264.7 cells using PAFAH antibody (15526-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, mouse spleen tissue, mouse liver tissue, RAW264.7
• Positive IHC detected in: human tonsillitis tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

15526-1-AP targets PAFAH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAFAH fusion protein Ag7227
• Full Name: phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 43-67 kDa
• GenBank Accession Number: BC038452
• Gene Symbol: PLA2G7
• Gene ID (NCBI): 7941
• RRID: AB_2878146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAFAH(platelet-activating factor acetylhydrolase, plasma) is also named as PLA2G7(phospholipase A2, group VII), Lp-PLA2 (Lipoprotein-associated phospholipase A2), LDL-PLA2, LDL-PLA and belongs to the AB hydrolase superfamily and lipase family. It is an enzyme which catalyzes the hydrolysis of acetyl ester at the sn-2 position of PAF. The family of PAF-Ahs consists of two intracellular isoforms (Ib and II), and one secreted isoform (plasma). Plasma PAF-AH is a monomeric polypeptide with a molecular weight of 45 kDa and it can show broad bands with a molecular weight of 43-67 kDa that it can be extensively N-glycosylated(PMID:12547653). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PAFAD), susceptibility to asthma (ASTHMA), susceptibility to atopic hypersensitivity (ATOPY).

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation.; Authors - Das Amrita A
mouse	WB	Nat Commun; VDAC2 enables BAX to mediate apoptosis and limit tumor development.; Authors - Hui San Chin
human,mouse	IF,IHC,WB	J Immunother Cancer; Inhibiting PLA2G7 reverses the immunosuppressive function of intratumoral macrophages and augments immunotherapy response in hepatocellular carcinoma; Authors - Feng Zhang
mouse	WB	J Extracell Vesicles; Characterization of protein, long noncoding RNA and microRNA signatures in extracellular vesicles derived from resting and degranulated mast cells.; Authors - Yuting Liang
mouse	IF,WB	Nat Metab; Elevated dietary ω-6 polyunsaturated fatty acids induce reversible peripheral nerve dysfunction that exacerbates comorbid pain conditions.; Authors - Jacob T Boyd ; KD Validated
human	IF	Cell Death Discov; Characterizing cellular heterogeneity in fibrotic hypersensitivity pneumonitis by single-cell transcriptional analysis.; Authors - Junyi Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sathyanarayani (Verified Customer) (05-12-2023)

I used this PAFAH antibody at a range of different dilutions from 1:300 to 1:1000 but unfortunately I could not detect any bands from this antibody when I used lysed mouse blood. The loading controls which were also from Proteintech produced strong clear bands on the western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Lysed mouse blood