PLA2G4B Polyclonal antibody

PLA2G4B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55074-1-AP

Synonyms

cPLA2 beta, HsT16992, Phospholipase A2 group IVB, PLA2G4B

---

Validation Data Gallery

• 

  WB analysis of human plasma using 55074-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 55074-1-AP (PLA2G4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 55074-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55074-1-AP (PLA2G4B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 55074-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55074-1-AP (PLA2G4B Antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: human plasma tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55074-1-AP targets PLA2G4B in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phospholipase A2, group IVB (cytosolic)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: ~88 kDa
• GenBank Accession Number: NM_001114633
• Gene Symbol: PLA2G4B
• Gene ID (NCBI): 100137049
• RRID: AB_2881261
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLA2G4B, also named as cPLA2-beta, is a member of the cytosolic phospholipase A2 protein family. It is calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids. PLA2G4B has five isoforms with MW 88 kDa, 100 kDa, 55 kDa and 114 kDa.