Tested Applications
Positive WB detected in | HaCaT cells, mouse skin tissue, rat skin tissue |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:600-1:2400 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
22632-1-AP targets Plakophilin 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Plakophilin 1 fusion protein Ag18153 Predict reactive species |
Full Name | plakophilin 1 (ectodermal dysplasia/skin fragility syndrome) |
Calculated Molecular Weight | 747 aa, 83 kDa |
Observed Molecular Weight | 70-75 kDa |
GenBank Accession Number | BC114571 |
Gene Symbol | PKP1 |
Gene ID (NCBI) | 5317 |
RRID | AB_3085701 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13835 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Plakophilin 1, also known as PKP1, B6P, is a member of the arm-repeat protein family. PKP1 contains arm-repeat (armadillo) domains, localizes to nuclei and cell desmosomes, and participates in linking cadherins to intermediate filaments in the cytoskeleton (PMID: 10747098). PKP1 contributes to epidermal morphogenesis and facilitates the formation of intermediate filaments. Mutations in PKP1 have been associated with the ectodermal dysplasia/skin fragility syndrome (PubMed:9326952, 10852826).
Protocols
Product Specific Protocols | |
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WB protocol for Plakophilin 1 antibody 22632-1-AP | Download protocol |
IHC protocol for Plakophilin 1 antibody 22632-1-AP | Download protocol |
IF protocol for Plakophilin 1 antibody 22632-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |