Plakophilin 1 Polyclonal antibody

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  WB analysis using 22632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22632-1-AP (PKP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 22632-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 22632-1-AP (PKP1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 22632-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using PKP1 antibody (22632-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Plakophilin 1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 22632-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PKP1, Plakophilin-1, B6P

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Tested Applications

• Positive WB detected in: HaCaT cells, mouse skin tissue, rat skin tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:600-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22632-1-AP targets Plakophilin 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Plakophilin 1 fusion protein Ag18153
• Full Name: plakophilin 1 (ectodermal dysplasia/skin fragility syndrome)
• Calculated Molecular Weight: 747 aa, 83 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC114571
• Gene Symbol: PKP1
• Gene ID (NCBI): 5317
• RRID: AB_3085701
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13835
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plakophilin 1, also known as PKP1, B6P, is a member of the arm-repeat protein family. PKP1 contains arm-repeat (armadillo) domains, localizes to nuclei and cell desmosomes, and participates in linking cadherins to intermediate filaments in the cytoskeleton (PMID: 10747098). PKP1 contributes to epidermal morphogenesis and facilitates the formation of intermediate filaments. Mutations in PKP1 have been associated with the ectodermal dysplasia/skin fragility syndrome (PubMed:9326952, 10852826).