PKN2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PKN2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14608-1-AP

Synonyms

PRKCL2, PRK2, PKN gamma, PAK2, Pak 2

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Validation Data Gallery

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  WB analysis of HEK-293 using 14608-1-AP

  WB result of PKN2 antibody (14608-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKN2 transfected HEK-293 cells.

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  WB analysis using 14608-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 14608-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 14608-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14608-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14608-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14608-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 14608-1-AP

  IP result of anti-PKN2 (IP:14608-1-AP, 4ug; Detection:14608-1-AP 1:1000) with COLO 320 cells lysate 2560 ug.

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  IHC staining of human colon cancer using 14608-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14608-1-AP (PKN2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human stomach using 14608-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14608-1-AP (PKN2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14608-1-AP

  Immunofluorescent analysis of HepG2 cells, using PKN2 antibody 14608-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 14608-1-AP

  Immunofluorescent analysis of Hela cells, using PKN2 antibody 14608-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 14608-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PKN2 (14608-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A2780 cells, K-562 cells, HeLa cells, HEK-293 cells, COLO 320 cells, SKOV-3 cells, HEK-293T cells
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human stomach tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, Hela cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

14608-1-AP targets PKN2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PKN2 fusion protein Ag6164
• Full Name: protein kinase N2
• Calculated Molecular Weight: 112 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC062620
• Gene Symbol: PKN2
• Gene ID (NCBI): 5586
• RRID: AB_10638487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancer Cell Int; Upregulation of SNTB1 correlates with poor prognosis and promotes cell growth by negative regulating PKN2 in colorectal cancer; Authors - Liya Liu
pig	WB,IHC	Reprod Domest Anim; miR-26a inhibits proliferation and promotes apoptosis in porcine immature Sertoli cells by targeting the PAK2 gene.; Authors - Maoliang Ran