PKM2-specific Monoclonal antibody, PBS Only

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  WB analysis of HEK-293 using 60268-1-Ig (same clone as 60268-1-PBS)

  WB result of PKM2 antibody (60268-1-Ig, 1:3000) with si-Control and si-PKM2 transfected HEK293 cells. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  WB analysis using 60268-1-Ig (same clone as 60268-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 60268-1-Ig (same clone as 60268-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60268-1-Ig (same clone as 60268-1-PBS)

  Western blot analysis of PK-M2-specific in various tissues and cell lines using Proteintech antibody 60268-1-Ig at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60268-1-Ig (same clone as 60268-1-PBS)

  Western blot analysis of PK-M2-specific in various lines using Proteintech antibody 60268-1-Ig at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 60268-1-Ig (same clone as 60268-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 60268-1-Ig (same clone as 60268-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 60268-1-Ig (same clone as 60268-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60268-1-Ig (same clone as 60268-1-PBS)

  IP result of anti-PKM2-specific (IP:60268-1-Ig, 5ug; Detection:15822-1-AP (PKM2-specific rabbit poly-antibody), 1:300) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60268-1-Ig (PKM2-specific antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60268-1-Ig (PKM2-specific antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:0 (under 10x lens). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:0 (under 40x lens). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKM2-specific antibody (60268-1-Ig, Clone: 1C11C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PKM2-specific Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 60268-1-PBS

Clone No.1C11C7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PKM, Pyruvate kinase PKM, EC:2.7.11.1, EC:2.7.10.2, EC:2.7.1.40

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Product Information

60268-1-PBS targets PKM2-specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: PKM pyruvate kinase, muscle
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: NM_002654
• Gene Symbol: PKM
• Gene ID (NCBI): 5315
• RRID: AB_2881388
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P14618
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms,the activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.