PKLR Polyclonal antibody

PKLR Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17580-1-AP

Synonyms

PK1, PKL, PKLR, PKR, PKRL, Pyruvate kinase 1, Pyruvate kinase isozymes R/L, pyruvate kinase, liver and RBC, R type/L type pyruvate kinase, Red cell/liver pyruvate kinase, RPK

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Validation Data Gallery

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  WB analysis of K-562 using 17580-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17580-1-AP (PKLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 17580-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17580-1-AP (PKLR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17580-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17580-1-AP (PKLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17580-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17580-1-AP (PKLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 17580-1-AP

  IP result of anti-PKLR (IP:17580-1-AP, 4ug; Detection:17580-1-AP 1:500) with K-562 cells lysate 3200ug.

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  IHC staining of human liver using 17580-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17580-1-AP (PKLR antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 17580-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17580-1-AP (PKLR antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse kidney tissue, mouse liver tissue, NIH/3T3 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17580-1-AP targets PKLR in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PKLR fusion protein Ag11096
• Full Name: pyruvate kinase, liver and RBC
• Calculated Molecular Weight: 574 aa, 62 kDa, 585 aa, 63 kDa
• Observed Molecular Weight: 58-62 kDa
• GenBank Accession Number: BC025737
• Gene Symbol: PKLR
• Gene ID (NCBI): 5313
• RRID: AB_2268201
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKLR(Pyruvate kinase isozymes R/L) is also named as PK1,PKL,which is a glycolytic enzyme that catalyzes the transphosphorylation from phosphoenolpyruvate (PEP) to ADP, yielding pyruvate and ATP. It is the last step of the glycolytic pathway and is essentially irreversible.It belongs to the pyruvate kinase family and There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) and pyruvate kinase deficiency of red cells (PKRD).It can form a homotetramer(PMID:11960989).