PIP5K1B Polyclonal antibody

• Featured Product
• KD/KO Validated

PIP5K1B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 12541-1-AP

Synonyms

PIP5K1 beta, PIP5K1B, PIP5KIbeta, Protein STM 7, PtdIns(4)P 5 kinase 1 beta, STM7

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Validation Data Gallery

• 

  WB analysis of human testis using 12541-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12541-1-AP (PIP5K1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12541-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12541-1-AP (PIP5K1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12541-1-AP

  IP result of anti-PIP5K1B (IP:12541-1-AP, 4ug; Detection:12541-1-AP 1:500) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human lung using 12541-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12541-1-AP (PIP5K1B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 12541-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12541-1-AP (PIP5K1B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 12541-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12541-1-AP (PIP5K1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12541-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12541-1-AP (PIP5K1B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 12541-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12541-1-AP (PIP5K1B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 12541-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12541-1-AP (PIP5K1B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 12541-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12541-1-AP (PIP5K1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 12541-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12541-1-AP (PIP5K1B antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human testis tissue, human brain tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human lung tissue, human heart tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

12541-1-AP targets PIP5K1B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIP5K1B fusion protein Ag3261
• Full Name: phosphatidylinositol-4-phosphate 5-kinase, type I, beta
• Calculated Molecular Weight: 549 aa, 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC030587
• Gene Symbol: PIP5K1B
• Gene ID (NCBI): 8395
• RRID: AB_10734598
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Clin Invest; Spastic paraplegia proteins spastizin and spatacsin mediate autophagic lysosome reformation.; Authors - Jaerak Chang
mouse	WB	EMBO J; PTPRN2 and PLCβ1 promote metastatic breast cancer cell migration through PI(4,5)P2-dependent actin remodeling.; Authors - Caitlin A Sengelaub
human	WB	EMBO J; Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKHM1 membrane cycling during autophagosome-lysosome fusion.; Authors - Takashi Baba ; KD Validated
human	WB	Cell Chem Biol; Targeting the PI5P4K Lipid Kinase Family in Cancer Using Covalent Inhibitors.; Authors - Sindhu Carmen Sivakumaren
		EMBO J; Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKMH1 membrane cycling during autophagosome-lysosome fusion.; Authors - Takashi Baba