PIP4K2A Polyclonal antibody

• Featured Product
• KD/KO Validated

PIP4K2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12469-1-AP

Synonyms

Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha, Phosphatidylinositol 5-phosphate 4-kinase type II alpha, Phosphatidylinositol 5-Phosphate 4-Kinase, EC:2.7.1.149, Diphosphoinositide kinase 2-alpha

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Validation Data Gallery

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  WB analysis of mouse brain using 12469-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12469-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12469-1-AP

  IP result of anti-PIP4K2A (IP:12469-1-AP, 4ug; Detection:12469-1-AP 1:500) with mouse brain tissue lysate 4400ug.

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  IHC staining of human spleen using 12469-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 12469-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 12469-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PIP4K2A antibody (12469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (black).

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  IF Staining of SH-SY5Y using 12469-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 12469-1-AP (PIP4K2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human spleen tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

12469-1-AP targets PIP4K2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIP4K2A fusion protein Ag3152
• Full Name: phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
• Calculated Molecular Weight: 406 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC018034
• Gene Symbol: PIP4K2A
• Gene ID (NCBI): 5305
• RRID: AB_10639518
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse,human	IF,IHC	Dev Cell; PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria interplay.; Authors - Archna Ravi
human	IF,IHC,IP,WB	Int J Biochem Cell Biol; A novel lncRNA lncSAMD11-1:1 interacts with PIP4K2A to promote endometrial decidualization by stabilizing FoxO1 nuclear localization; Authors - Mei Zhang
human,mouse	WB,IHC,IF	Sci Adv; PI5P4Kα supports prostate cancer metabolism and exposes a survival vulnerability during androgen receptor inhibition; Authors - Joanna Triscott ; KD Validated