PIM2 Polyclonal antibody

PIM2 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25865-1-AP

Synonyms

pim 2 oncogene, Pim 2h, PIM2

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Validation Data Gallery

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  IP experiment of Raji using 25865-1-AP

  IP result of anti-PIM2 (IP:25865-1-AP, 4ug; Detection:25865-1-AP 1:300) with Raji cells lysate 1320ug.

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  IHC staining of mouse liver using 25865-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25865-1-AP (PIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IP detected in: Raji cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

25865-1-AP targets PIM2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIM2 fusion protein Ag23130
• Full Name: pim-2 oncogene
• Calculated Molecular Weight: 311 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC018111
• Gene Symbol: PIM2
• Gene ID (NCBI): 11040
• RRID: AB_2880275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIM2 was first identified in T and B cell lymphomas in mice and is a member of a serine/threonine kinase family of proto-oncogenes, which also includes PIM1 and PIM3 (PMID:24505470). They share high sequence homology at the amino acid level; PIM1 and PIM2 are 61% identical and PIM1 and PIM3 are 71% identical (PMID:34503111). The PIM2 protein plays an important role in promoting cell survival and preventing apoptosis. PIM2 is mainly expressed in lymphoid and brain tissues. In human cells, the PIM2 kinase has only two isoforms (34 and 41 kDa), but in mice there are there (34, 37, and 40 kDa), and the 34 kDa isoform has been the main focus of many studies because it plays a crucial role in tumor progression (PMID:33854618).

Publications

Species	Application	Title
human	WB	Phytomedicine; Abrine promotes cell proliferation and inhibits apoptosis of interleukin-1β-stimulated chondrocytes via PIM2/VEGF signalling in osteoarthritis.; Authors - Yong Meng