PILRA Polyclonal antibody

PILRA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11818-1-AP

Synonyms

Cell surface receptor FDF03, FDF03, Inhibitory receptor PILR alpha, PILRA, PILRα

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Validation Data Gallery

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  WB analysis using 11818-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11818-1-AP (PILRA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11818-1-AP targets PILRA in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PILRA fusion protein Ag2385
• Full Name: paired immunoglobin-like type 2 receptor alpha
• Calculated Molecular Weight: 303 aa, 34 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC017812
• Gene Symbol: PILRA
• Gene ID (NCBI): 29992
• RRID: AB_2163648
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Paired receptors consist of highly related activating and inhibitory receptors and are widely involved in the regulation of the immune system. Paired immunoglobulin-like type 2 receptor alpha (PILRα) and PILRβ are paired receptors bearing highly similar extracellular ligand-binding domains but divergent intracellular signaling domains. PILRα is the immune inhibitory receptor possessing an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain enabling it to deliver inhibitory signals. Binding of PILRα to its ligand CD99 is involved in immune regulation. PILRα can also recognize other ligands, PILR-associating neural protein (PANP), and HSV-1 glycoprotein B (HSV-1 gB). PILRα has a calculated molecular weight of 34 kDa. It has been reported that PILRα migrates at 44-50 kDa due to glycosylation (PMID: 10903717; 10660620). The other band of about 61 kDa detected by this antibody may present a heavily glycosylated form of PILRα.