PI3 Kinase p110 Delta Monoclonal antibody

PI3 Kinase p110 Delta Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G11C6

Cat no : 67964-1-Ig

Synonyms

p110D, PI3 kinase subunit delta, PI3K delta, PIK3CD, PtdIns 3 kinase subunit delta

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Validation Data Gallery

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  WB analysis of LNCaP using 67964-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67964-1-Ig (PIK3CD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67964-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67964-1-Ig (PIK3CD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of LNCaP using 67964-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using PIK3CD antibody (67964-1-Ig, Clone: 2G11C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, Jurkat cells
• Positive IF/ICC detected in: LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67964-1-Ig targets PI3 Kinase p110 Delta in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PI3 Kinase p110 Delta fusion protein Ag26995
• Full Name: phosphoinositide-3-kinase, catalytic, delta polypeptide
• Calculated Molecular Weight: 1044 aa, 120 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC132919
• Gene Symbol: PIK3CD
• Gene ID (NCBI): 5293
• RRID: AB_2918715
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.