PI3 Kinase p110 Alpha Polyclonal antibody

PI3 Kinase p110 Alpha Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 21890-1-AP

Synonyms

p110 alpha, PI3 Kinase p110 Alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PI3K p110(alpha), PI3-kinase p110 subunit alpha, PIK3CA, PtdIns 3 kinase subunit alpha

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Validation Data Gallery

• 

  WB analysis of human brain using 21890-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21890-1-AP (PI3K p110(alpha) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21890-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21890-1-AP (PI3K p110(alpha) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 21890-1-AP

  IP result of anti-PI3 Kinase p110 Alpha (IP:21890-1-AP, 4ug; Detection:21890-1-AP 1:300) with mouse heart tissue lysate 3600 ug.

• 

  IHC staining of human lung cancer using 21890-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21890-1-AP (PI3K p110(alpha) antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 21890-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21890-1-AP (PI3K p110(alpha) antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21890-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21890-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 21890-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21890-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 21890-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21890-1-AP (PI3K p110(alpha) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 21890-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21890-1-AP (PI3K p110(alpha) antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 21890-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 21890-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 21890-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 21890-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 21890-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 21890-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue, Jurkat cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 20 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 21890-1-AP is PI3 Kinase p110 Alpha Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PI3 Kinase p110 Alpha fusion protein Ag16586
• Full Name: phosphoinositide-3-kinase, catalytic, alpha polypeptide
• Calculated Molecular Weight: 1068 aa, 124 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC113601
• Gene Symbol: PIK3CA
• Gene ID (NCBI): 5290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

Publications

Species	Application	Title
rat	WB	Food Funct; l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways; Authors - Ling Lin
human	WB	J Agric Food Chem; β-Sitosterol-D-glucoside from sweet potato exerts an anti-breast cancer activity by upregulating microRNA-10a and PI3K/Akt signaling pathway.; Authors - Heshan Xu
human	WB	J Hematol Oncol; Long non-coding RNA-SNHG7 acts as a target of miR-34a to increase GALNT7 level and regulate PI3K/Akt/mTOR pathway in colorectal cancer progression.; Authors - Yang Li
mouse	WB,IHC	Sci Rep; NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice.; Authors - Shu Yang
human	WB	J Exp Clin Cancer Res; LINC01296/miR-26a/GALNT3 axis contributes to colorectal cancer progression by regulating O-glycosylated MUC1 via PI3K/AKT pathway.; Authors - Bing Liu
mouse	WB	Sci Rep; Hypoxia-induced ANGPTL4 sustains tumour growth and anoikis resistance through different mechanisms in scirrhous gastric cancer cell lines.; Authors - Koichi Baba

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