PIK3C2B Polyclonal antibody

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  WB analysis using 24788-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24788-1-AP (PIK3C2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24788-1-AP

  IP result of anti-PIK3C2B (IP:24788-1-AP, 4ug; Detection:24788-1-AP 1:1500) with HeLa cells lysate 1240 ug.

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  IP experiment of HeLa using 24788-1-AP

  IP result of anti-PIK3C2B (IP:24788-1-AP, 5ug; Detection:24788-1-AP 1:800) with HeLa cells lysate 2500ug.

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  IHC staining of human skeletal muscle using 24788-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24788-1-AP (PIK3C2B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 24788-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24788-1-AP (PIK3C2B Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 24788-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24788-1-AP (PIK3C2B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PIK3C2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 24788-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C2 PI3K, PI3K C2 beta, PIK3C2B

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

24788-1-AP targets PIK3C2B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIK3C2B fusion protein Ag20474
• Full Name: phosphoinositide-3-kinase, class 2, beta polypeptide
• Calculated Molecular Weight: 1634 aa, 185 kDa
• Observed Molecular Weight: 160-185 kDa
• GenBank Accession Number: BC132876
• Gene Symbol: PIK3C2B
• Gene ID (NCBI): 5287
• RRID: AB_2879725
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00750
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human		Anal Chem; Pseudotargeted MS Method for the Sensitive Analysis of Protein Phosphorylation in Protein Complexes.; Authors - Jiawen Lyu
human	WB	Cell Rep; ALKBH5 causes retinal pigment epithelium anomalies and choroidal neovascularization in age-related macular degeneration via the AKT/mTOR pathway; Authors - Ru-Xu Sun