PIGX Polyclonal antibody

PIGX Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17120-1-AP

Synonyms

PIG-X, PIG X, Phosphatidylinositol-glycan biosynthesis class X protein

---

Validation Data Gallery

• 

  WB analysis using 17120-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17120-1-AP (PIGX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: COLO 320 cells, HEK-293K cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17120-1-AP targets PIGX in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIGX fusion protein Ag10808
• Full Name: phosphatidylinositol glycan anchor biosynthesis, class X
• Calculated Molecular Weight: 217aa,25 kDa; 258aa,29 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC022542
• Gene Symbol: PIGX
• Gene ID (NCBI): 54965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIGX encodes a 258-amino acid ER-resident type I transmembrane protein with a large lumenal domain. It acts by stabilizing the mannosyltransferase PIGM. PIGX might function as a cyclic dimeric GMP phosphodiesterase.