PIGK Recombinant antibody

PIGK Recombinant Antibody for WB, ELISA
Cat No. 84568-3-RR
Clone No.241989D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

hGPI8, GPI-anchor transamidase, GPI8, GPI transamidase, EC:3.-.-.-

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inA549 cells, K-562 cells, HeLa cells, HT-1080 cells, U-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84568-3-RR targets PIGK in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PIGK fusion protein Ag3497 Predict reactive species
Full Name phosphatidylinositol glycan anchor biosynthesis, class K
Calculated Molecular Weight 45 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberBC026186
Gene Symbol PIGK
Gene ID (NCBI) 10026
RRIDAB_3672072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ92643
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphatidylinositol glycan, class K (PIGK), is a crucial member of the glycosyl-phosphatidylinositol transamidase (GPIT) protein complex that attaches a diverse group of macromolecules to the plasma membrane of eukaryotes. The human PIGK gene is involved in the key step of transferring GPI-anchor to the respective protein molecules in the plasma membrane (PMID: 22824918). Endogenous PIGK has 2 isoforms, 45 kDa and 36 kDa (PMID: 34193731).

Protocols

Product Specific Protocols
WB protocol for PIGK antibody 84568-3-RRDownload protocol
Standard Protocols
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