Piezo1 (extracellular domain) Polyclonal antibody

• Featured Product
• KD/KO Validated

Piezo1 (extracellular domain) Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 28511-1-AP

Synonyms

FAM38A, PIEZO1, Protein FAM38A, Piezo-type mechanosensitive ion channel component 1, Membrane protein induced by beta-amyloid treatment

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Validation Data Gallery

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  WB analysis of HeLa using 28511-1-AP

  WB result of Piezo1 (extracellular domain) antibody (28511-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and Piezo1 (extracellular domain) knockout HeLa cells.

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  WB analysis of Hepa1-6 using 28511-1-AP

  N/A

• 

  WB analysis using 28511-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28511-1-AP (Piezo1 (extracellular domain) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28511-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28511-1-AP (Piezo1 (extracellular domain) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 28511-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28511-1-AP (PIEZO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28511-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28511-1-AP (PIEZO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of THP-1 using 28511-1-AP

  Immunofluorescent analysis of THP-1 cells using Piezo1 (extracellular domain) antibody (28511-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Hepa1-6 cells, HepG2 cells, hTERT-RPE1 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

28511-1-AP targets Piezo1 (extracellular domain) in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Piezo1 (extracellular domain) fusion protein Ag29858
• Full Name: family with sequence similarity 38, member A
• Calculated Molecular Weight: 286 kDa
• Observed Molecular Weight: 280-300 kDa
• GenBank Accession Number: BC008073
• Gene Symbol: Piezo1/FAM38A
• Gene ID (NCBI): 9780
• RRID: AB_2881161
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells which influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as PIEZO1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Fam38A also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in gene encoding PIEZO1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1.

Publications

Species	Application	Title
mouse	WB,IF	Glia; Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes; Authors - Yue Wan ; KD Validated
human	WB,IF,IP	FASEB J; Mechanical stretch activates piezo1 in caveolae of alveolar type I cells to trigger ATP release and paracrine stimulation of surfactant secretion from alveolar type II cells.; Authors - Kathrin Diem ; KD Validated
human	WB	JCI Insight; Mechanosensitive Piezo1 channels mediate renal fibrosis.; Authors - Xiaoduo Zhao
human	WB,CoIP	Heliyon; The function of Piezo1 in hepatoblastoma metastasis and its potential transduction mechanism; Authors - Xiaoshuo Ye ; KD Validated
mouse	WB,IF	Neuron; Microglial Piezo1 senses Aβ fibril stiffness to restrict Alzheimer's disease; Authors - Jin Hu
human	WB,IHC	Mol Neurobiol; Exosomal circZNF800 Derived from Glioma Stem-like Cells Regulates Glioblastoma Tumorigenicity via the PIEZO1/Akt Axis; Authors - Ning Zhang ; KD Validated