PICK1 Polyclonal antibody, PBS Only

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  WB analysis using 10983-2-AP (same clone as 10983-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10983-2-AP (PICK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10983-2-AP (same clone as 10983-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10983-2-AP (PICK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 10983-2-AP (same clone as 10983-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10983-2-AP (PICK1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 10983-2-AP (same clone as 10983-2-PBS)

  IP result of anti-PICK1 (IP:10983-2-AP, 4ug; Detection:10983-2-AP 1:2500) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 10983-2-AP (same clone as 10983-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10983-2-AP (PICK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 10983-2-AP (same clone as 10983-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10983-2-AP (PICK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 10983-2-AP (same clone as 10983-2-PBS)

  Immunofluorescent analysis of HEK-293 cells using 10983-2-AP (PICK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PICK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 10983-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

10983-2-PBS targets PICK1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PICK1 fusion protein Ag1443
• Full Name: protein interacting with PRKCA 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC017561
• Gene Symbol: PICK1
• Gene ID (NCBI): 9463
• RRID: AB_2164525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NRD5
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Protein interacting with C kinase 1 (PICK1) was first cloned as a PKC-binding partner through yeast two hybrid system. PICK1 acts as a critical regulator of membrane receptors' subcellular trafficking to modulate neural processes such as learning and memory, and is widely expressed in brain, testis, heart, lung, liver, kidney and muscle. It probably binds to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence, for instance, PICK1 is a critical mediator of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking in neural synapses. PICK1 expression on D-serine release and glutamate transport in astrocytes suggests a potential implication of PICK1 in the progression of amyotrophic lateral sclerosis (ALS). PICK1 may also participate in breast cancer development through inhibition of TGF-β signaling.