PIAS3 Polyclonal antibody

• Featured Product
• KD/KO Validated

PIAS3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 13486-1-AP

Synonyms

ZMIZ5, Protein inhibitor of activated STAT protein 3, EC:2.3.2.-, E3 SUMO-protein transferase PIAS3, E3 SUMO-protein ligase PIAS3

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Validation Data Gallery

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  WB analysis of HepG2 using 13486-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13486-1-AP (PIAS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13486-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13486-1-AP (PIAS3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 13486-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13486-1-AP (PIAS3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13486-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13486-1-AP (PIAS3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13486-1-AP

  Immunofluorescent analysis of HepG2 cells using 13486-1-AP (PIAS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

13486-1-AP targets PIAS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIAS3 fusion protein Ag4304
• Full Name: protein inhibitor of activated STAT, 3
• Calculated Molecular Weight: 628 aa, 68 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC030556
• Gene Symbol: PIAS3
• Gene ID (NCBI): 10401
• RRID: AB_2164224
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIAS3(E3 SUMO-protein ligase PIAS3) is a protein of 583 amino acid, has a molecular mass of 68 kDa and belongs to a family of proteins that includes several other members(PMID:11060035). PIAS3 is an inhibitor of activated STAT3 and binds microphthalmia-associated transcription factor, which is a key DNA-binding protein, in rat basophilic leukemia cells and mouse melanocytes(PMID:11709556). The PIAS3 antibody detects PIAS3 (68 kDa) as well as its sumoylated form (85 kDa) in some normal mouse tissues, breast and brain tumor cell lines(PMID:20371673).

Publications

Species	Application	Title
human,mouse	WB	Biochim Biophys Acta Mol Cell Res; AMPK induces PIAS3 mediated SUMOylation of E3 ubiquitin ligase Smurf1 impairing osteogenic differentiation and traumatic heterotopic ossification; Authors - Jie Chen
human	WB	Mol Med Rep; Role of SUMOylation of STAT1 in tubular epithelial‑mesenchymal transition induced by high glucose; Authors - Cunyang Gu
human	IHC	Int J Mol Sci; Resveratrol Enhances Temozolomide Efficacy in Glioblastoma Cells through Downregulated MGMT and Negative Regulators-Related STAT3 Inactivation; Authors - Moli Wu
human	WB,IHC	Pharmacol Res; PIAS3 promotes ferroptosis by regulating TXNIP via TGF-β signaling pathway in hepatocellular carcinoma; Authors - Wenfang Bao ; KD Validated
rat	WB	Free Radic Biol Med; PIAS3 acts as a zinc sensor under zinc deficiency and plays an important role in myocardial ischemia/reperfusion injury; Authors - Huanhuan Zhao