PIAS2 Recombinant antibody

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  WB analysis of mouse testis using 84189-4-RR

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84189-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84189-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84189-4-RR against Human PIAS2 were performed. The affinity constant is 0.28 nM.

PIAS2 Recombinant Antibody for WB, ELISA

Cat No. 84189-4-RR

Clone No.241474G8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

E3 SUMO-protein ligase PIAS2, DIP, DAB2-interacting protein, ARIP3, Androgen receptor-interacting protein 3

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Tested Applications

• Positive WB detected in: K-562 cells, mouse testis tissue, HeLa cells, HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84189-4-RR targets PIAS2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PIAS2 fusion protein Ag9032
• Full Name: protein inhibitor of activated STAT, 2
• Calculated Molecular Weight: 63 kDa, 69 kDa
• Observed Molecular Weight: 63-68 kDa
• GenBank Accession Number: BC015190
• Gene Symbol: PIAS2
• Gene ID (NCBI): 9063
• RRID: AB_3671742
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75928
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIAS2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. PIAS2 also plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. PIAS2 has 3 isoforms produced by alternative splicing with the MW of 68 kDa, 63 kDa and 45 kDa.