PIAS2 Recombinant antibody
PIAS2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
241474G8
Cat no : 84189-4-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, mouse testis tissue, HeLa cells, HEK-293T cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 84189-4-RR is PIAS2 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | PIAS2 fusion protein Ag9032 |
Full Name | protein inhibitor of activated STAT, 2 |
Calculated Molecular Weight | 63 kDa, 69 kDa |
Observed Molecular Weight | 63-68 kDa |
GenBank Accession Number | BC015190 |
Gene Symbol | PIAS2 |
Gene ID (NCBI) | 9063 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PIAS2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. PIAS2 also plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. PIAS2 has 3 isoforms produced by alternative splicing with the MW of 68 kDa, 63 kDa and 45 kDa.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PIAS2 antibody 84189-4-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |