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  • KD/KO Validated

PI4KA Polyclonal antibody

PI4KA Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12411-1-AP

Synonyms

PI4 kinase alpha, PI4K ALPHA, pi4K230, PI4KA, PIK4, PIK4CA, PtdIns 4 kinase alpha



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, human brain tissue, MCF-7 cells, NIH-3T3 cells (RNAi), rat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

12411-1-AP targets PI4KA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PI4KA fusion protein Ag3062
Full Name phosphatidylinositol 4-kinase, catalytic, alpha
Calculated molecular weight 2044 aa, 231 kDa
Observed molecular weight 231 kDa
GenBank accession numberBC018120
Gene symbol PI4KA
Gene ID (NCBI) 5297
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).

Protocols

Product Specific Protocols
WB protocol for PI4KA antibody 12411-1-APDownload protocol
IHC protocol for PI4KA antibody 12411-1-APDownload protocol
IP protocol for PI4KA antibody 12411-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Authors - Jay Xiaojun Tan
  • KD Validated
humanWB

Science

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Authors - Shun Nagashima
  • KD Validated
humanWB,IF

Brain

Biallelic PI4KA variants cause a novel neurodevelopmental syndrome with hypomyelinating leukodystrophy.

Authors - Edgard Verdura
humanIF

EMBO J

Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.

Authors - Zhengming Wu
human,mouseWB,IHC,IF

Cell Rep

A Forward Genetic Screen Targeting the Endothelium Reveals a Regulatory Role for the Lipid Kinase Pi4ka in Myelo- and Erythropoiesis.

Authors - Safiyyah Ziyad
  • KD Validated
humanWB

Biomaterials

Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells.

Authors - Ming Gao
  • KD Validated