PI4KA Polyclonal antibody

• Featured Product
• KD/KO Validated

PI4KA Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 12411-1-AP

Synonyms

PI4 kinase alpha, PI4K ALPHA, pi4K230, PI4KA, PIK4, PIK4CA, PtdIns 4 kinase alpha

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Validation Data Gallery

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  WB analysis of MCF-7 using 12411-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH-3T3 cells (RNAi) using 12411-1-AP

  WB result of anti- PI4KA (12411-1-AP) with NIH-3T3 cells (RNAi).

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  WB analysis using 12411-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12411-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12411-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12411-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12411-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12411-1-AP

  IP Result of anti-PI4KA (IP:12411-1-AP, 5ug; Detection:12411-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human placenta using 12411-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12411-1-AP (PI4KA Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 12411-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12411-1-AP (PI4KA Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, human brain tissue, MCF-7 cells, NIH-3T3 cells (RNAi), rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 8 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

12411-1-AP targets PI4KA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PI4KA fusion protein Ag3062
• Full Name: phosphatidylinositol 4-kinase, catalytic, alpha
• Calculated molecular weight: 2044 aa, 231 kDa
• Observed molecular weight: 231 kDa
• GenBank accession number: BC018120
• Gene symbol: PI4KA
• Gene ID (NCBI): 5297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).

Publications

Species	Application	Title
human	WB	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan ; KD Validated
human	WB	Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima ; KD Validated
human	WB,IF	Brain; Biallelic PI4KA variants cause a novel neurodevelopmental syndrome with hypomyelinating leukodystrophy.; Authors - Edgard Verdura
human	IF	EMBO J; Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.; Authors - Zhengming Wu
human,mouse	WB,IHC,IF	Cell Rep; A Forward Genetic Screen Targeting the Endothelium Reveals a Regulatory Role for the Lipid Kinase Pi4ka in Myelo- and Erythropoiesis.; Authors - Safiyyah Ziyad ; KD Validated
human	WB	Biomaterials; Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells.; Authors - Ming Gao ; KD Validated