Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, human brain tissue, MCF-7 cells, NIH-3T3 cells (RNAi), rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12411-1-AP targets PI4KA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3062 Product name: Recombinant human PI4KA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1745-2044 aa of BC018120 Sequence: YLAKFKVKRCGVSELEKEGLRCRSDSEDECSTQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQSCFLSNRSRTYDMIQYYQNDIPY Predict reactive species |
| Full Name | phosphatidylinositol 4-kinase, catalytic, alpha |
| Calculated Molecular Weight | 2044 aa, 231 kDa |
| Observed Molecular Weight | 231 kDa |
| GenBank Accession Number | BC018120 |
| Gene Symbol | PI4KA |
| Gene ID (NCBI) | 5297 |
| RRID | AB_2268237 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42356 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PI4KA antibody 12411-1-AP | Download protocol |
| IHC protocol for PI4KA antibody 12411-1-AP | Download protocol |
| IP protocol for PI4KA antibody 12411-1-AP | Download protocol |
| WB protocol for PI4KA antibody 12411-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PI4KA Polyclonal antibody (Cat# 12411-1-AP) has 14 total citations across journals including Nature, Science, Theranostics, Biomaterials, Brain, EMBO Journal, Cell Reports, Communications Chemistry, PLoS Pathogens, FASEB Journal, Journal of Virology, Cell Stress & Chaperones, and PLoS ONE. Research fields encompass lysosomal repair, mitochondrial division, leukemia therapy, neurodevelopmental disorders, lipid kinase signaling, hepatitis C virus assembly, renal tumor progression, synaptic transmission, and phagosome biogenesis.
| Species | Application | Title |
|---|---|---|
Science Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.
| ||
Theranostics Targeting PI4KA sensitizes refractory leukemia to chemotherapy by modulating the ERK/AMPK/OXPHOS axis
| ||
Biomaterials Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells.
| ||
Brain Biallelic PI4KA variants cause a novel neurodevelopmental syndrome with hypomyelinating leukodystrophy. | ||
EMBO J Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB. |
Reviews
What customers say
The antibody received a 5-star rating from Patricia Panikulam (June 2024), who used it at a 1:2000 dilution for Western Blot and Immunofluorescence on immune cells. She reported that it works well with 30 µg of cell lysate. Overall, the single review reflects positive performance and ease of use across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Patricia (Verified Customer) (06-16-2024) | Works well with 30ug of cell lysate
|
