PI4KA Polyclonal antibody

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  WB analysis of NIH-3T3 cells (RNAi) using 12411-1-AP

  WB result of anti- PI4KA (12411-1-AP) with NIH-3T3 cells (RNAi).

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  WB analysis of MCF-7 using 12411-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12411-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12411-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12411-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12411-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12411-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12411-1-AP

  IP result of anti-PI4KA (IP:12411-1-AP, 4ug; Detection:12411-1-AP 1:3000) with mouse brain tissue lysate 1080 ug.

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  IHC staining of human placenta using 12411-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12411-1-AP (PI4KA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 12411-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12411-1-AP (PI4KA Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 12411-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12411-1-AP (PI4KA Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 12411-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PI4KA antibody (12411-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PI4KA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12411-1-AP

Publications(12)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PIK4, PI4-kinase alpha, PI4K-alpha, Phosphatidylinositol 4-Kinase III alpha, Phosphatidylinositol 4-kinase alpha

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, human brain tissue, MCF-7 cells, NIH-3T3 cells (RNAi), rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

12411-1-AP targets PI4KA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PI4KA fusion protein Ag3062
• Full Name: phosphatidylinositol 4-kinase, catalytic, alpha
• Calculated Molecular Weight: 2044 aa, 231 kDa
• Observed Molecular Weight: 231 kDa
• GenBank Accession Number: BC018120
• Gene Symbol: PI4KA
• Gene ID (NCBI): 5297
• RRID: AB_2268237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P42356
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).

Publications

Species	Application	Title
human	WB	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan ; KD Validated
human	WB	Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima ; KD Validated
human	WB,IF	Brain; Biallelic PI4KA variants cause a novel neurodevelopmental syndrome with hypomyelinating leukodystrophy.; Authors - Edgard Verdura
human	IF	EMBO J; Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.; Authors - Zhengming Wu
human,mouse	WB,IHC,IF	Cell Rep; A Forward Genetic Screen Targeting the Endothelium Reveals a Regulatory Role for the Lipid Kinase Pi4ka in Myelo- and Erythropoiesis.; Authors - Safiyyah Ziyad ; KD Validated
human	WB	Biomaterials; Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells.; Authors - Ming Gao ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Patricia (Verified Customer) (06-16-2024)

Works well with 30ug of cell lysate

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: immune cells