PI3 Kinase p110 Alpha Polyclonal antibody

PI3 Kinase p110 Alpha Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 27921-1-AP

Synonyms

p110 alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PI3K p110(alpha), PIK3CA, PtdIns 3 kinase subunit alpha

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Validation Data Gallery

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  WB analysis using 27921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27921-1-AP (PI3K p110(alpha) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27921-1-AP (PI3 Kinase p110 Alpha antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse brain tissue, NIH/3T3 cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below

Product Information

27921-1-AP targets PI3 Kinase p110 Alpha in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PI3 Kinase p110 Alpha fusion protein Ag27539
• Full Name: phosphoinositide-3-kinase, catalytic, alpha polypeptide
• Calculated Molecular Weight: 1068 aa, 124 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC113601
• Gene Symbol: PIK3CA
• Gene ID (NCBI): 5290
• RRID: AB_2918135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

Publications

Species	Application	Title
rat	WB	J Ethnopharmacol; Xiao-Luo-Wan treats propylthiouracil-induced goiter with hypothyroidism in rats through the PI3K-AKT/RAS pathways based on UPLC/MS and network pharmacology.; Authors - Shi Dong
mouse,human	WB	Heliyon; Anticancer potential of grifolin in lung cancer treatment through PI3K/AKT pathway inhibition; Authors - Li Wang
mouse	WB	JCI Insight; Regulatory T-cell and neutrophil extracellular traps interaction contributes to the development of immunosuppression in sepsis; Authors - Yuxin Shi
human	WB	Anticancer Drugs; STK214947, a novel indole alkaloids, inhibits HeLa and SK-HEP-1 cells survival and EMT process by blocking the Notch3 and Akt signals; Authors - Zihan Xu
human	WB	J Immunother; Vitexin Inhibits TNBC Progression and Metastasis by Modulating Macrophage Polarization Through EGFR Signaling; Authors - Yufeng Lin
mouse	WB	Eur J Pharmacol; Novel Applications of Metformin in the Treatment of Septic Myocardial Injury Based on Metabolomics and Network Pharmacology; Authors - Xingyu Li