PI3 Kinase p85 Beta Monoclonal antibody, PBS Only

PI3 Kinase p85 Beta Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B3B6

Cat no : 67644-1-PBS

Synonyms

p85, p85 BETA, P85B, PI3 Kinase p85 Beta, PI3 kinase subunit p85 beta, PI3K regulatory subunit beta, PIK3R2

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Validation Data Gallery

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  WB analysis using 67644-1-Ig (same clone as 67644-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67644-1-Ig (PI3 Kinase p85 Beta antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

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  WB analysis using 67644-1-Ig (same clone as 67644-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67644-1-Ig (PIK3R2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67644-1-Ig (same clone as 67644-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67644-1-Ig (PIK3R2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67644-1-Ig (same clone as 67644-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67644-1-Ig (PIK3R2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67644-1-Ig (same clone as 67644-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PI3 Kinase p85 Beta antibody (67644-1-Ig, Clone: 2B3B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

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Product Information

67644-1-PBS targets PI3 Kinase p85 Beta in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PI3 Kinase p85 Beta fusion protein Ag14369
• Full Name: phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• Calculated Molecular Weight: 728 aa, 82 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC014170
• Gene Symbol: PI3 Kinase p85 Beta
• Gene ID (NCBI): 5296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PI3 Kinase p85 is also named as PIK3R2 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).