PI3 Kinase p110 Beta Monoclonal antibody, PBS Only

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  WB analysis using 67121-1-Ig (same clone as 67121-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67121-1-Ig (same clone as 67121-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67121-1-Ig (same clone as 67121-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67121-1-Ig (same clone as 67121-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67121-1-Ig (same clone as 67121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67121-1-Ig (PIK3CB antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67121-1-Ig (same clone as 67121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67121-1-Ig (PIK3CB antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67121-1-Ig (same clone as 67121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67121-1-Ig (same clone as 67121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67121-1-Ig (same clone as 67121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 67121-1-Ig (same clone as 67121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67121-1-Ig (same clone as 67121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67121-1-Ig (same clone as 67121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67121-1-Ig (same clone as 67121-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PI3 Kinase p110 Beta antibody (67121-1-Ig, Clone: 1B2A1 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

PI3 Kinase p110 Beta Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67121-1-PBS

Clone No.1B2A1

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PI3K p110(beta), PIK3CB, PI3K, PI3 kinase subunit beta, p110 BETA

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Product Information

67121-1-PBS targets PI3 Kinase p110 Beta in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PI3 Kinase p110 Beta fusion protein Ag17505
• Full Name: phosphoinositide-3-kinase, catalytic, beta polypeptide
• Calculated Molecular Weight: 1070 aa, 123 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC114432
• Gene Symbol: PI3 Kinase p110 Beta
• Gene ID (NCBI): 5291
• RRID: AB_2882424
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P42338
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002).