PI3 Kinase p110 Alpha Recombinant antibody

PI3 Kinase p110 Alpha Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B4

Cat no : 82796-4-RR

Synonyms

PI3K p110(alpha), PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, p110 alpha

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Validation Data Gallery

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  WB analysis using 82796-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82796-4-RR (PIK3CA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82796-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82796-4-RR against Human PI3 Kinase p110 Alpha were performed. The affinity constant is 0.639 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, COLO 320 cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82796-4-RR targets PI3 Kinase p110 Alpha in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PI3 Kinase p110 Alpha fusion protein Ag16586
• Full Name: phosphoinositide-3-kinase, catalytic, alpha polypeptide
• Calculated Molecular Weight: 1068 aa, 124 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC113601
• Gene Symbol: PIK3CA
• Gene ID (NCBI): 5290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).