PI3 Kinase p110 Alpha Recombinant antibody
PI3 Kinase p110 Alpha Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1B4
Cat no : 82796-4-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, COLO 320 cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
82796-4-RR targets PI3 Kinase p110 Alpha in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | PI3 Kinase p110 Alpha fusion protein Ag16586 |
Full Name | phosphoinositide-3-kinase, catalytic, alpha polypeptide |
Calculated Molecular Weight | 1068 aa, 124 kDa |
Observed Molecular Weight | 110 kDa |
GenBank Accession Number | BC113601 |
Gene Symbol | PIK3CA |
Gene ID (NCBI) | 5290 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).
Protocols
Product Specific Protocols | |
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WB protocol for PI3 Kinase p110 Alpha antibody 82796-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |