PHYHIPL Polyclonal antibody

PHYHIPL Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 16937-1-AP

Synonyms

KIAA1796, PHYHIPL

---

Validation Data Gallery

• 

  WB analysis using 16937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16937-1-AP (PHYHIPL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse brain using 16937-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 16937-1-AP (PHYHIPL antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, mouse testis tissue, rat testis tissue
• Positive IHC detected in: mouse brain tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16937-1-AP targets PHYHIPL in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHYHIPL fusion protein Ag10435
• Full Name: phytanoyl-CoA 2-hydroxylase interacting protein-like
• Calculated Molecular Weight: 376 aa, 43 kDa
• Observed Molecular Weight: 39 kDa and 42-45 kDa
• GenBank Accession Number: BC011268
• Gene Symbol: PHYHIPL
• Gene ID (NCBI): 84457
• RRID: AB_2163761
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHYHIPL, a kind of mitochondrial protein, is associated with energy production, oxidative stress and organelle dynamics. It is reported that global ischemia is related to the change of PHYHIPL protein level. Compared with the control group, the level of APOE (may be involved in abnormal regulation of mitochondrial function) is significantly increased after ischemic stimulation, while the APOE genotype has a effect on PHYHIPL protein level. 16937-1-AP detects 39 kDa isoform and 42-45 kDa isoform in SDS-PAGE. (PMID: 21878944)